New non-invasive methodology allows for early diagnosis of diseases such as osteoporosis and Alzheimer’s

New non-invasive methodology allows for early diagnosis of diseases such as osteoporosis and Alzheimer’s

Fluorescent Dye

A new non-invasive methodology, developed by UGR researchers, allows for early diagnosis of diseases such as osteoporosis and Alzheimer’s

Scientists from the University of Granada have developed a new fluorescent dye capable of detecting, in a single test lasting 20 minutes, the presence of phosphate and biothiol inside living cells. This scientific breakthrough could contribute significantly to the early diagnosis of diseases such as osteoporosis, Alzheimer’s, type 2 diabetes, and prostate cancer, since abnormal levels of both substances are associated with these diseases.

In a paper published in the academic journal Chemistry - A European Journal, the researchers, who work at the UGR’s Department of Physical Chemistry, illustrate the design, synthesis and photophysical studies carried out with this new fluorescent dye.

The main author behind the study, Luis Crovetto González, explains: “We have successfully managed to create, for the first time, a dual function dye capable of detecting both substances in the same test. Until now, this procedure has been conducted using two separate fluorescent dyes and/or two separate tests.”

The continuation of a patent from 2014

In 2014, the same research group patented a new non-invasive method that allows for the measurement, in real-time, of concentration levels of phosphate ions inside living cells. This new dye that they have developed is, in effect, the continuation of this previous research and subsequent patent.

The importance of being able to measure phosphate ions stems precisely from the fact that these measurements can be employed to assess the bioavailability of drugs used to treat certain diseases, among others, osteoporosis.

At present, the only available method for calculating the concentration levels of phosphates found inside the osteoblasts (the precursor cells of bone) is invasive, employing radioactive phosphorous, the use of which carries serious risks.

As such, the methodology developed by the UGR researchers marks a considerable scientific breakthrough. Given its significance, their paper appeared on the cover of the latest edition of Chemisty – A European Journal.

The paper is dedicated to Professor Carlos Orte Martínez from the UGR’s Department of Physical Chemistry, who passed away in March 2015.


Frontispiece: New Dual Fluorescent Probe for Simultaneous Biothiol and Phosphate Bioimaging, Sandra Resa, Angel Orte, Delia Miguel, Jose M. Paredes, Virginia Puente-Muñoz, Rafael Salto, Maria D. Giron, Maria J. Ruedas-Rama, Juan M. Cuerva, Jose M. Alvarez-Pez, Luis Crovetto

Chemistry - A European Journal, 2015, 21, 14772-14779

You can read the full paper here:


Luis Crovetto González

Department of Physical Chemistry

Phone: +34 958249473 or +34 958243825


Image: Beginning of an experiment in which there is no fluorescence, how it first appears (owing to the biothiols) and how the fluorescence changes owing to the phosphates.